PDF | Cariotipo, bandeo R-replicativo, roedor, cromosomas, idiograma. Idiograma de la guagua de cola Dynomis branickii con bandas R-replicativas; diseñado en guagua de cola, pacarana, guagua loba, entre otras. Cariotipo con bandeo G. Ailuropoda melanoleuca. Bandeo Cromosómico en mamíferos Cariotipo con bandeo G de E. glacialis. de bandeo cromosómico G, C, Q, R y NOR se estableció su cariotipo 2n= . definidos para el cariotipo del cariomorfo A de S granatensis con bandas G.
|Published (Last):||21 August 2014|
|PDF File Size:||4.58 Mb|
|ePub File Size:||4.12 Mb|
|Price:||Free* [*Free Regsitration Required]|
Cytogenetic characterization of the silverside fish Odontesthes regia Humboldt, Teleostei: Atherinopsidae from Iquique, Bwndeo. Atherinopsidae de Iquique, Chile. This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization FISHto locate ribosomal genes rDNA.
The karyotype is composed of one submetacentric pair pair 116 subtelocentric pairs pairs 2 to 17and 7 acrocentric pairs pairs 18 to With the exception of pair 1 it was not possible to classify caritoipo homologous chromosomes accurately because differences in chromosome size were too slight between adjacent pairs.
The distribution of C-banded heterochromatin allowed for a more accurate matching of the majority of chromosomes of the subtelocentric series. The great importance of conservation biology and the need to develop new tools that allow understanding ecosystems, phyletic relationship among taxa and identification of new species, has encouraged scientists to perform new karyological cariotipp in fishes.
Cytogenetic studies of fishes from the South American continent have displayed a considerable expansion in the last years. Among these studies, the contribution of the late Dr. A recent paper by Winkler et al. Odontesthes regia Humboldt, Teleostei: Although the family Atherinopsidae includes species 13 generaonly 17 species have been cytogenetically studied to date Table 1demonstrating that cytogenetic studies in this group are still scarce.
Chromosome bbandeo of Atheriniformes Tabla 1. As part of a project to study the cytogenetic biodiversity of the Chilean ichthyofauna, this paper is the first report of the karyotype of O. A sample comprising five specimens 2 females and 3 males of O. C-bands were obtained according to Sumner Mitotic chromosomes were photographed using a digital camera and images were digitally processed with Adobe Photoshop v. The karyogram was constructed with chromosomes organized in size-decreasing order.
Chromosomes were classified following Levan et al.
The representative karyogram for the species is shown in Fig. The karyotype of this species consists of one submetacentric pair pair 116 subtelocentric pairs pairs 2 to 17and 7 acrocentric pairs pair s 18 to With the exception of pair 1, it was not possible to carry out an accurate classification of chromosomes as homologous pairs due to their similar morphology and slight differences in chromosome size.
According to the most recent review of fish chromosome data, A recent study Miya et al.
Despite the conserved diploid number in the genus Odontesthesa clear difference in chromosome formulae is observed between O. If for comparative purposes we consider the subtelocentric chromosomes of O. C-banded heterochromatin is distributed in a pericentromeric position in all chromosomes except a large positive interstitial band near the centromere in pair 6, and a large telomeric segments in chromosome pairs 3 and 7. Such distribution of C-banded heterochromatin allows for a more accurate, tentatively homologous chromosome pairing in the majority of chromosomes of the subtelocentric series Fig.
Cytogenetic characteristics of Odontesthes regia. A Giemsa stained karyotype. Above pair 1 the same chromosome pair stained with silver nitrate to show the Ag-NORs black dots.
B C-banded karyotypes Figure 1. B Cariotipos de bandeo C. As far as ribosomic cistrons are concerned, the most frequent localization of NORs in fish chromosomes is terminal on a single chromosome pair Galetti et al. The use of FISH with 18S rDNA probing is a technique extremely well suited to characterize a species; it allows researchers to know whether differences in Ag-NOR number are related only with the differential activities of these regions or with different numbers of NORs in the genome of different specimens Gornung et al.
Metaphase of Odontesthes regia showing chromosomes after fluorescent in situ hybridization with the 18S rDNA gene. Arrows point out the 18S-bearing chromosomes Figure 3. Las flechas indican los cromosomas que llevan el 18S. We are gratefull to anonymous reviewers who contributed to the improvement cxriotipo the final version of the manuscript. Karyotypes of Diplomystid and certain loricarioid catfishes.
Diversidad genética y evolución cariotípica de los mamíferos
Cariotipos de Aplochiton zebra y Aplochiton taeniatus Pisces: Chromosomes of two species of atherinoid fishes. Bulletin of the National Science Museum Ser.
A karyotype study on two species of freshwater caeiotipo transplanted into Japan. Cytotaxonomic considerations on Hoplias lacerdae Pisces, Erythrinidae. Brazilian Journal of Genetics 1: Nucleolar organizer regions, their function and polyploidy in fishes. Journal Obshchej Biology 6: Karyology of three galaxiid fishes Galaxias maculates, G.
Cytogenetics and Cell Genetics Cytochemical studies on the nature of NOR nucleolus organizer region silver stainability.
Cellular and Molecular Biology Cytotaxonomic studies on Chilean galaxiid fishes. Polymorphic nature of nucleolus organizer regions in fishes. Karyotypes of Basilichthys microlepidotus and B. Characterization of eight species of Anostomidae Cypriniformes fish on the basis of nucleolar organizing regions.
Cytogenetics | Protocol (Translated to Spanish)
Karyotype and nucleolar organizer regions of Pygocentrus cariba Humboldt, Pisces: Serrasalminae from Caicara del Orinoco, Venezuela, Interciencia 30 1: Silver-staining and heteromorphism of chromosomal nucleolus organizer regions in North American cyprinid fishes.
Controlled silver staining of Nucleolus Organizer Regions with a protective colloidal developer: Silver staining as an indicator of active ribosomal genes. A study of the silver staining significance in mitotic NORs. Da tab ase of fish chromosomes. Westarp Wissenschaften, Magdeburg, Germany, pp. Karyology of three species of Eastern North American Atherinid fishes. Nomenclature for centrometric position on chromosomes.
Acriotipo study on nucleolar silver staining in plant cells. The role of argyrophylic proteins in nucleolar physiology. Major patterns of higher teleostean phylogenies: Molecular Phylogenetics and Evolution Fishes of the world. Cytogenetic characterization of hybrids offspring between Colossoma macropomum Cuvier, and Piaractus brachypomus Cuvier, from Caicara del Orinoco, Venezuela. Stock-specifc gg in the number and chromosome positions of the nucleolar regions in Arctic charr Salvelinus alpinus.
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proceedings of the National Academy of Sciences Ag-NOR proteins are present when transcription is impaired. Biology of the Cell 5: Cytogenetical characterization of Odontesthes bonariensis Pisces, Atherinidaean Argentine species introduced in Italy.
A simple technique for demonstrating centromeric heterocromatin. Experimental Cell Research Karyotype analysis in three species of the genus Chirostoma Atheriniformes: Chrnosomnye tschisla ryboobrasnych i ryb. Evoljuzionnaja kariologiya ryb, pp. Karyotypes of three South East Pacific flounder species of the family Paralichthyidae.